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A serial dilution is any dilution in which the concentration decreases by the same factor in each successive step. In serial dilutions, you multiply the dilution factors for each step. The dilution factor or the dilution is the initial volume divided by the final volume. #DF = V_i/V_f# For example, if you add a 1 mL sample to 9 mL of diluent to get 10 mL of solution, #DF = V_i/V_f# = #(1'mL')/(10'mL') = 1/10#.
This is a 1:10 dilution. Example 1 What is the dilution factor if you add 0.2 mL of a stock solution to 3.8 mL of diluent? #V_f# = 0.2 mL + 3.8 mL = 4.0 mL #DF = V_i/V_f# = #(0.2'mL')/(4.0'mL') = 1/20#.
This is a 1:20 dilution. Example 2 If you did the above dilution four times, what would be the final dilution factor? Solution 2 Remember that serial dilutions are always made by taking a set quantity of the initial dilution and adding it successively to tubes with the same volume.
So you multiply each successive dilution by the dilution factor. You would transfer 0.2 mL from Tube 1 to 3.8 mL of diluent in Tube 2 and mix. Then transfer 0.2 mL from Tube 2 to 3.8 mL of diluent in Tube 3 and mix. Repeat the process until you have four tubes. The dilution factor after four dilutions is #DF = 1/20 × 1/20 × 1/20 × 1/20 = 1/160000# = 1:160 000 If the concentration of the original stock solution was 100 µg/µL, the concentration in Tube 4 would be 100 µg/µL × #1/160000# = 6.25 × 10⁻⁴ µg/µL Hope this helps.
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Many downloads like Zillatube 1.3 Activation Code may also include a serial number, cd key or keygen. If this is the case then it's usually included in the full crack download archive itself. If you are still having trouble finding Zillatube 1.3 Activation Code after simplifying your search term then we highly recommend using the alternative. Zillatube returned 5 download results. Full version downloads hosted on high speed servers! Zillatube also includes Crack Serial & Keygen. Slimming to or eliminating registration code crack zillatube the sports video, or panting, of a management. Registration code crack zillatube. Many downloads like Zillatube may also include a crack, serial number, unlock code, cd key or keygen (key generator). To create more accurate search results for Zillatube Key try to exclude using commonly used keywords such as: crack, download, serial, keygen, torrent, warez, etc. Serial Key For Zillatube. Protect your applications by serial key registration. To send and receive serial data through a remote serial port on the PC. Zillatube unlock code Free Download,Zillatube unlock code Software Collection Download. TV on PC Pro 8. Unlock code for rockware zillatube code. Keygen.
Compare the serial number with the one found on your Order Confirmation email from your Trend Micro program. If the serial number is the same, check expiry date if it has been extended. If not, prepare the following details below then contact our Technical Support.
Hello everyone, I found this thread while googling.
A serial dilution is any dilution in which the concentration decreases by the same factor in each successive step. In serial dilutions, you multiply the dilution factors for each step. The dilution factor or the dilution is the initial volume divided by the final volume. #DF = V_i/V_f# For example, if you add a 1 mL sample to 9 mL of diluent to get 10 mL of solution, #DF = V_i/V_f# = #(1'mL')/(10'mL') = 1/10#.
This is a 1:10 dilution. Example 1 What is the dilution factor if you add 0.2 mL of a stock solution to 3.8 mL of diluent? #V_f# = 0.2 mL + 3.8 mL = 4.0 mL #DF = V_i/V_f# = #(0.2'mL')/(4.0'mL') = 1/20#.
This is a 1:20 dilution. Example 2 If you did the above dilution four times, what would be the final dilution factor? Solution 2 Remember that serial dilutions are always made by taking a set quantity of the initial dilution and adding it successively to tubes with the same volume.
So you multiply each successive dilution by the dilution factor. You would transfer 0.2 mL from Tube 1 to 3.8 mL of diluent in Tube 2 and mix. Then transfer 0.2 mL from Tube 2 to 3.8 mL of diluent in Tube 3 and mix. Repeat the process until you have four tubes. The dilution factor after four dilutions is #DF = 1/20 × 1/20 × 1/20 × 1/20 = 1/160000# = 1:160 000 If the concentration of the original stock solution was 100 µg/µL, the concentration in Tube 4 would be 100 µg/µL × #1/160000# = 6.25 × 10⁻⁴ µg/µL Hope this helps.